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Porvair Filtration Group has announced that the Childrens Research Institute at the Medical College of Wisconsin (www.chw.org) has used the company's Chromatrap^TM technology to develop more efficient and more precise Chromatin Immunoprecipitation (ChIP) assays.

The Children's Research Institute is renowned for its state-of-the-art pediatric studies.  The CRI's translational research programs are targeted to find life-saving discoveries and cures in the diseases that affect children and interventions that enhance quality of life for children and families living with chronic health conditions.

Over the last few months - Dr Ronald N Hines, Professor of Pediatrics, Pharmacology & Toxicology at the CRI has compared the efficacy of Chromatrap^TM with traditional bead based ChIP technologies in his research into known regulatory elements controlling ontogeny of important human drug metabolizing enzymes, such as CYP3A4.  He commented "I believe the bead-based system gave us a relatively small signal to noise ratio such that even minor deviations caused by variation in the efficiency of the bead system (for whatever reason) resulted in considerable variation in the assay".  He added "By comparison I think the most substantial advantage of the Chromatrap system is more efficient and less variable trapping of the immunoprecipitated chromatin such that the signal to noise ratio is larger and influenced less by minor variations in trapping efficiency".

The Chromatrap^TM spin column approach offers significant advantages compared to methods based on agarose or magnetic beads, which involve many steps of separation, pipetting and re-suspension. The BioVyon^TM protein A discs used in Chromatrap^TM spin columns have a proprietary internal structure, composed of pores that have inner surfaces specifically designed to maximize chromatin capture efficiency. Porvair's proprietary disc base material is chemically inert, reducing incidences of non-specific binding. All chromatin capture reactions take place inside the rigid porous disc; therefore there is no need for the many repetitive stages of suspension, collection and resuspension by pipetting. Additionally no pre-blocking step is required. The Chromatrap spin column protocol allows washing steps that are fast, efficient and less prone to error and the 'flow through' characteristics of the column ensure excellent sample mixing and washing during the assay.

For further information please visit www.chromatrap.com or contact Porvair Filtration Group on +44-1489-864330 /  kevin.quinlan@chromatrap.com.

Porvair Filtration Group is an international leader in the development and supply of materials and products for filtration and separation applications. Their expertise in a wide range of media and the dedication of their design, manufacturing, sales, test and research teams ensure they are at the forefront of filtration technology, delivering world class performance to the Life Science, Aerospace, Defence, Nuclear, Energy, Chemical Process, Industrial Process, Water Treatment and Printing  markets. With offices and manufacturing sites located in the UK, Europe and the USA combined with a world-wide network of market representatives and distributors, they pride themselves on their continuous innovation and research to meet global demands.

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