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Dionex Demonstrates Simplified Sample Preparation Method for HPAE-PAD Analysis
publication date: Oct 11, 2010
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author/source: Dionex
Dionex announces a new method that simplifies sample pretreatment for small-scale peptide N-glycosidase F (PNGase F) digests that use protein denaturation. Application Update 176: Preparation of Peptide N-Glycosidase F Digests for HPAE-PAD Analysis demonstrates that this new pretreatment method makes samples amenable to high-performance anion-exchange chromatography with pulsed amperometric detection (HPAE-PAD) oligosaccharide analysis with the CarboPac® PA200 column, thereby making it possible to monitor the oligosaccharide contentº of a glycoprotein. This approach overcomes the limitations of using traditional size-exclusion columns with low- to medium-molecular weight cut-off resins for sample pretreatment. Most size-exclusion methods are time consuming, require large amounts of sample, and run the risk of losing charged sugars. The biotechnology industry has already produced a number of glycoprotein therapeutics and more are being investigated for their therapeutic potential. The companies that manufacture these products must understand and monitor the oligosaccharide content of the glycoprotein to ensure that it is consistent from one lot of therapeutic to another. HPAE-PAD is one of the tools used to study and monitor glycoprotein oligosaccharides. Dionex (NASDAQ:DNEX) is a global leader in the manufacturing and marketing of liquid chromatography and sample preparation systems, consumables, and software for chemical analysis. The company's systems are used worldwide in environmental analysis and by the life sciences, chemical, petrochemical, food and beverage, power generation, and electronics industries. Our expertise in applications and instrumentation helps analytical scientists to evaluate and develop pharmaceuticals, establish environmental regulations, and produce better industrial products. For further information please visit www.dionex.com CarboPac is a registered trademark of Dionex Corporation. |
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