HPLC-Tip: Degassing of the mobile phase
publication date: Dec 30, 2009
A released gas in mobile phases during analysis causes noisy baselines and pressure fluctuations which affect sensitivity and reproducibility. It also severely compromises the use of fluorescence-, refractive-index- or UV-detectors (particularly at wavelengths below 210 nm). As a consequence effective mobile phase degassing must be employed for many analyses.
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