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Look deep into your samples, without the need for sectioning
publication date: Jan 3, 2012
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author/source: OLYMPUS EUROPA HOLDING GMBH
 Olympus has released the new XLPLN25XSVMP SCALEVIEW
25x objective lens (NA 1.0) for the deep imaging of thick biological samples. The
revolutionary SCALEVIEW approach was developed in collaboration with the RIKEN
Brain Institute in Japan, where it allowed researchers to create highly
accurate 3D structural representations of brain tissue. Until now, the use of
such samples has been limited by the effects of tissue opacity and light
scattering. To counter this, the new objective has a
super-long-working-distance of 4mm and works alongside Olympus's new
SCALEVEW-A2 clearing agent, which renders samples nearly translucent while
preserving fluorescent signals. Image quality, sharpness and brightness are
then maximised as the objective is optimised to match the refractive index
properties of the clearing agent. When used as part of a complete Olympus
FluoView FV1000MPE multiphoton system, the new objective makes it possible to
peer deeper into samples than ever before, allowing you to generate truly
insightful results from intact specimens.The SCALEVIEW 25x objective and SCALEVIEW-A2 reagent are designed to boost the capabilities of multiphoton microscopy, allowing accurate reconstructions up to a depth of 4mm to be generated using fluorescent markers. Previously, the investigation of thick or complex samples such as brain tissue had required that analysis be carried out using thin tissue sections. Mechanical slicing of tissue into very thin sections requires elaborate protocols and challenging data reconstruction methods to visualize exactly how the slices fit together. By clearing formalin-fixed tissues and allowing light to pass through the sample, the SCALEVIEW-A2 reagent minimises the need for sectioning, allowing the user to generate insightful data that more accurately reflects the true internal structure of a complex specimen. Using the technique, true 3D representations can be created, without the need for complex interpolation, predictive algorithms or guesswork. To maximise the advantages provided by the SCALEVIEW-A2 clearing solution, Olympus has released the SCALEVIEW 25x objective, which has been specifically designed for deep imaging. This dedicated multiphoton objective with an ultra-long working distance enables the high-precision imaging of transparent biological specimens to a depth of 4mm. The objective is equipped with a correction collar and is optimised to work best with the SCALEVIEW-A2 reagent (refractive index 1.38), facilitating the production of detailed, crisp images. As part of a complete system, the new SCALEVIEW approach integrates seamlessly with Olympus's FluoView FV1000MPE multiphoton microscopes and the FV10-ASW software v3.1, providing the power to visualise 3-dimensional structures at unprecedented depths in morphologically intact tissue. For more information visit www.microscopy.olympus.eu |
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