Sartobind® Phenyl for removal
of hydrophobic contaminants
Sartorius Stedim
Biotech, a leading international laboratory and process technology provider, is
launching the first commercially available membrane adsorber for hydrophobic
interaction chromatography (HIC).Sartobind
Phenyl membrane adsorbers allow increased flow rates to ensure faster processes
and can be used for process upgrades in purification and polishing of therapeutic proteins.
Sartobind
Phenyl provides the advantages of an HIC ligand adsorber, while showing the
specificity and behavior of a conventional HIC matrix. The hydrophobic phenyl ligand is covalently attached to the >3 µm
cellulose membrane. Target molecules bind to the membrane with similar
binding capacities and at the same buffer conditions as with conventional HIC
columns. However, the flow rates can be increased at least 10-fold to allow a faster process upgrade in purification and polishing of therapeutic proteins. The resolution is at
least as good as that obtained with comparable phenyl columns.
With a binding
capacity of 0.4 mg/cm² (15 mg/ml), Sartobind Phenyl can be used for antibody
medium scale capture. Its key application is removal of hydrophobic aggregates that
pose the risk of unwanted immune system response when patients are treated with
therapeutic proteins. Detergent removal and large molecule / bioparticle
purification are further application options for HIC membranes.
The
membrane adsorber is easy to handle, suitable for single and batch use and scalable from the 3 ml Sartobind nano version to the Sartobind 150 ml
capsule. It will soon also be scalable to the Jumbo 5 l unit.
Sartobind® Phenyl for removal
of hydrophobic contaminants