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Chromatography Articles and Application Notes

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Catch up on the latest chromatography articles and application notes


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Latest News

  Analysis of Nitrate and Nitrite in Milk
May 23, 2012
Analysis of Nitrate and Nitrite in MilkThermo Fisher Scientific Inc. is pleased to announce a new application for the determination of nitrite and nitrate in milk by ion chromatography (IC) with suppressed conductivity detection using in-line sample preparation. Application Note 279: Time Savings and Improved Reproducibility of Nitrate and Nitrite Ion Chromatography Determination in Milk Samples describes the method where the prepared sample is separated on the high-capacity Thermo Scientific Dionex IonPac TM AS20 hydroxide-selective anion-exchange column to resolve nitrite and nitrate from the remaining sample components...
  HPLC-CAD Improves Determination of Triterpenes in Gota Kola
May 22, 2012
Determination of Triterpenes in Gota KolaThermo Fisher Scientific is pleased to announce a new method that uses high-performance liquid chromatography (HPLC) combined with charged aerosol detection (CAD) to enable improved quantitation of triterpene content in gotu kola. Low-wavelength UV detection commonly used for triterpene analysis often results in strongly sloping baselines, and interferences from minor components can adversely affect quantitation. Customer Application Note (CAN) 111: Determination of Triterpenes in Centella asiatica (Gotu Kola) by HPLC-CAD demonstrates that charged aerosol detection minimizes these interferences and improves the baseline, thereby enabling improved quantitation of triterpenes in gotu kola...
  Malvern team presents ‘Nanoparticle characterization by light scattering’ poster at ACS Colloids 2012 symposium
May 22, 2012
Nanoparticle characterization by light scatteringMalvern Instruments light scattering specialists Dr Ana Morfesis and Dr Ulf Nobbmann, will present a poster on ‘Nanoparticle characterization by light scattering' as part of the 86th ACS Colloid and Surface Science Symposium being held at the University of Baltimore in Maryland,  USA, June 10-13, 2012. Their poster will be displayed from 5.30 until 7.30 pm on Monday June 11 in a session held in the exhibition area, where delegates will also be able to visit the Malvern booth to find out more about the company's full range of systems for nanoparticle characterization, including the market leading Zetasizer Nano.
  Determination of Ginsenosides in Panax Ginseng - New method announced by Thermo Fisher Scientific
May 15, 2012
Determination of Ginsenosides in Panax GinsengThermo Fisher Scientific is pleased to announce a new method that uses the Thermo Scientific Dionex Corona CAD Charged Aerosol Detector to provide a reliable alternative to low-wavelength UV detection. Customer Application Note (CAN) 112: Determination of Ginsenosides in Panax ginseng by HPLC-CAD demonstrates that charged aerosol detection does not overrespond to strong chromophores, thereby reducing interferences. Baseline slopes are not as pronounced or as variable, making peak area determinations more reliable...
  Analysis of Organic Acids in Wastewater
May 14, 2012
Thermo Fisher Scientific App Note 291Thermo Fisher Scientific is pleased to announce a new combined method for the accurate determination of organic acids in a wastewater sample. Application Note 291: Determination of Organic Acids in Wastewater Using Ion-Exclusion Chromatography and On-Line Carbonate Removal demonstrates that ion-exclusion chromatography excludes the inorganic anions present in the sample. A Thermo Scientific Dionex Carbonate Removal Device (CRD 200) is also needed to remove carbonate on line. Inorganic anions and carbonate can interfere when determining organic acids in wastewater using anion-exchange chromatography...
  New Malvern download with detailed Zetasizer specifications helps users match system with application
May 9, 2012
Malvern Zetasizer particle characterization systemsIn line with a policy of continuous improvement, Malvern Instruments has reviewed, verified and published updated specifications for the Zetasizer series of dynamic light scattering (DLS) particle characterization systems, all fully backed by measurements on real samples. Full updated specification details are available online...
  Analysis of Carbohydrates in Coffee in 10 mins
May 2, 2012
Application Note 280: Carbohydrate in CoffeeThermo Fisher Scientific is pleased to announce a new approach using high-performance anion-exchange ( HPAE) chromatography with pulsed amperometric detection (PAD) for the determination of carbohydrates in extracts from instant coffee and green coffee beans. Application Note 280: Carbohydrate in Coffee: AOAC Method 995.13 vs a New Fast Ion Chromatography Method compares two methods: the Association of Analytical Chemists ( AOAC) official method 995.13 and a fast method using the CarboPac TM SA10 colum...
  Determination of Steviol Glycoside in Sweeteners
Apr 17, 2012
Determination of Steviol Glycoside in SweetenersThermo Fisher Scientific Inc. is pleased to announce an economical new method that uses high-performance liquid chromatography ( HPLC) with charged aerosol and UV detections to determine steviol glycosides in consumer sweeteners. Application Note 293: Steviol Glycoside Determination by HPLC with Charged Aerosol and UV Detections Using the Acclaim Trinity P1 Column demonstrates that only 3-9 mL of mobile phase/sample are required, compared to 25-60 mL/sample for the Joint FAO/WHO Commission on Food Additives ( JECFA) and U.S. Pharmacopeia (USP) methods, respectively. The reduction in mobile phase use saves both time and resources during mobile phase preparation and also reduces waste...
  HPLC Analysis of 6 Active Components of Caulis Lonicerae
Apr 13, 2012
Thermo Fisher Scientific pplication Note 299Thermo Fisher Scientific Inc. is pleased to announce an efficient, comprehensive HPLC QC method using an Acclaim Phenyl-1 Column for the separation of all six main active components of Caulis Lonicerae, an important traditional Chinese medicine. To regulate this natural treatment used for acute fever, headache, respiratory infection, and epidemic diseases, the Pharmacopoeia of the People's Republic of China (PPRC) requires two different methods, which combined only identify two main target components...
  Simple, Fast Determination of Phytic Acid in Soybeans and Black Sesame Seeds
Apr 12, 2012
Fast Determination of Phytic Acid in Soybeans and Black Sesame SeedsThermo Fisher Scientific Inc. is pleased to announce a simple, fast (< 10 min per injection) isocratic method for the determination of phytic acid (as phytate) in soybeans and black sesame seeds by using a hydroxide-selective column combined with a Reagent-FreeTM ion chromatography (RFICTM) system, as described in Application Note 295, Determination of Phytic Acid in Soybeans and Black Sesame Seeds. The RFIC system eliminated the required time and possible errors associated with manual eluent preparation and the method was shown to be accurate and reproducible for these two samples, and should be applicable to other food samples...
 
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